Shannon Strenger was working as a surgical assistant while she attended nursing school. Her job involved taking tissue samples to the histology lab, where a pathologist prepared them for examination. "I found this process so fascinating that I eventually changed fields," says Strenger, a histotechnician at St. Joseph's Hospital, a member of the HealthEast Care System.
Preparing The Specimen
Whenever tissue such as a gall bladder, a lymph node or a tumor is removed or biopsied, the histotechnician prepares very thin sections of the specimen for microscopic examination.
This preparation involves five basic steps:
•Grossing and fixation. The pathologist examines, describes and trims the specimen to the right size. Then it is placed in a chemical solution to prevent decomposition.
•Processing. Water is removed from the tissue and replaced by melted paraffin wax. This allows the tissue to be cut into thin slices.
•Embedding. The wax-permeated tissue is placed in a larger wax block to facilitate slicing.
•Sectioning. The tissue is cut into very thin sections using a delicate instrument called a microtome and placed on a glass slide.